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gateway vector gal4 system  (TaKaRa)


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    TaKaRa gateway vector gal4 system
    ( A ) Yeast-two-hybrid assay showing interaction between SPL/NZZ and MADS-domain TFs (SEP3, STK, AG). The controls are shown in the . Interactions are tested on media depleted of histidine (-W -L -H), or histidine and adenine (-W -L -H -A). For each interaction on the different media, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( B, C ) Yeast-three-hybrid assay showing the formation of SPL/NZZ, STK and SEP3 complex, by fusing SPL/NZZ either to the <t>GAL4</t> BD ( B ) or to the TFT ( C ). Interactions are tested on a medium depleted of histidine (-W -L -H -A). For each interaction, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( D, E ) pSEP3::SEP3:GFP reporter line showing SEP3 accumulation in ovules at stage 2-I ( D ) and 2-III ( E ). ( F, G ) pSTK::STK:GFP reporter line in ovules at stage 2-I ( F ) and 2-II ( G ). ( H, I ) pAG::AG:GFP reporter line in ovules at stage 2-I ( H ) and 2-II ( I ). Abbreviations: nu= nucella; ch= chalaza; fu= funiculus; ii= inner integument; oi= outer integument; pl= placenta. Scale bar = 20 μm.
    Gateway Vector Gal4 System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3975 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gateway+vector+gal4+system/pGADT7+AD+Vector/bio_rxiv__2025__03__11__641985-315-18-25
    Average 99 stars, based on 3975 article reviews
    gateway vector gal4 system - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "SPOROCYTELESS/NOZZLE acts together with MADS-domain transcription factors to regulate an auxin-dependent network controlling the Megaspore Mother Cell development"

    Article Title: SPOROCYTELESS/NOZZLE acts together with MADS-domain transcription factors to regulate an auxin-dependent network controlling the Megaspore Mother Cell development

    Journal: bioRxiv

    doi: 10.1101/2025.03.11.641985

    ( A ) Yeast-two-hybrid assay showing interaction between SPL/NZZ and MADS-domain TFs (SEP3, STK, AG). The controls are shown in the . Interactions are tested on media depleted of histidine (-W -L -H), or histidine and adenine (-W -L -H -A). For each interaction on the different media, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( B, C ) Yeast-three-hybrid assay showing the formation of SPL/NZZ, STK and SEP3 complex, by fusing SPL/NZZ either to the GAL4 BD ( B ) or to the TFT ( C ). Interactions are tested on a medium depleted of histidine (-W -L -H -A). For each interaction, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( D, E ) pSEP3::SEP3:GFP reporter line showing SEP3 accumulation in ovules at stage 2-I ( D ) and 2-III ( E ). ( F, G ) pSTK::STK:GFP reporter line in ovules at stage 2-I ( F ) and 2-II ( G ). ( H, I ) pAG::AG:GFP reporter line in ovules at stage 2-I ( H ) and 2-II ( I ). Abbreviations: nu= nucella; ch= chalaza; fu= funiculus; ii= inner integument; oi= outer integument; pl= placenta. Scale bar = 20 μm.
    Figure Legend Snippet: ( A ) Yeast-two-hybrid assay showing interaction between SPL/NZZ and MADS-domain TFs (SEP3, STK, AG). The controls are shown in the . Interactions are tested on media depleted of histidine (-W -L -H), or histidine and adenine (-W -L -H -A). For each interaction on the different media, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( B, C ) Yeast-three-hybrid assay showing the formation of SPL/NZZ, STK and SEP3 complex, by fusing SPL/NZZ either to the GAL4 BD ( B ) or to the TFT ( C ). Interactions are tested on a medium depleted of histidine (-W -L -H -A). For each interaction, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( D, E ) pSEP3::SEP3:GFP reporter line showing SEP3 accumulation in ovules at stage 2-I ( D ) and 2-III ( E ). ( F, G ) pSTK::STK:GFP reporter line in ovules at stage 2-I ( F ) and 2-II ( G ). ( H, I ) pAG::AG:GFP reporter line in ovules at stage 2-I ( H ) and 2-II ( I ). Abbreviations: nu= nucella; ch= chalaza; fu= funiculus; ii= inner integument; oi= outer integument; pl= placenta. Scale bar = 20 μm.

    Techniques Used: Y2H Assay, Serial Dilution, Hybrid Assay

    Related Articles

    Clone Assay:

    Article Title: A molecular framework controlling style morphology in Brassicaceae
    Article Snippet: The Y2H assays were performed at 28°C in the yeast strain AH109 (Clontech) using the co-transformation technique. .. Coding sequences were cloned into Gateway vector GAL4 system (pGADT7 and pGBKT7; Clontech) passing through pDONR207 (Life Technologies). .. Strength of interaction was tested on selective yeast synthetic dropout medium (YSD) lacking Leu (L), Trp (W), Adenine (A), and His (H), supplemented with different concentrations of 3-aminotriazole (Sigma-Aldrich).

    Article Title: SPOROCYTELESS/NOZZLE acts together with MADS-domain transcription factors to regulate an auxin-dependent network controlling the Megaspore Mother Cell development
    Article Snippet: .. AG and STK full-length CDS and SPL/NZZ and SEP3Δ shorter versions (SPL/NZZΔ and SEP3Δ respectively) were cloned into Gateway vector GAL4 system (pGBKT7 and pGADT7, Clontech; pTFT1 ). ..

    Article Title: TEOSINTE BRANCHED1 Regulates Inflorescence Architecture and Development in Bread Wheat ( Triticum aestivum )
    Article Snippet: .. These open reading frames were cloned into the Gateway vector GAL4 system ( pGADT7 and pGBKT7 ; Clontech) passing through pDONR207 (Life Technologies, Thermo Fischer Scientific). ..

    Article Title: BPC transcription factors and a Polycomb Group protein confine the expression of the ovule identity gene SEEDSTICK in Arabidopsis.
    Article Snippet: All rights reserved Yeast two-hybrid assay The two-hybrid assays were performed at 28°C in the yeast strain AH109 (Clontech). .. The coding sequences of BPC4, BPC6 and SVP were cloned into pDONR207 (Life Technologies) and successively transferred to the Gateway vector GAL4 system (pGADT7 and pGBKT7; Clontech). ..

    Article Title: A noncanonical auxin-sensing mechanism is required for organ morphogenesis in Arabidopsis
    Article Snippet: The Y2H assays were performed at 28°C in the yeast strain AH109 (Clontech) using the cotransformation technique ( ). .. Coding sequences were cloned into pGAD424 and pGBKT9 vectors (Clontech) using the SmaI and PstI sites or cloned into the Gateway vector GAL4 system (pGADT7 and pGBKT7; Clontech) passing through pDONR207 (Life Technologies). ..

    Article Title: MADS-Box and bHLH Transcription Factors Coordinate Transmitting Tract Development in Arabidopsis thaliana
    Article Snippet: The two-hybrid assays were performed at 22°C in the yeast strain AH109 (Clontech), using the cotransformation technique ( ). .. The coding sequences of CESTA , STK , and BEE1 were cloned in the Gateway vector GAL4 system (pGADT7 and pGBKT7; Clontech) passing through pDONOR207 (Life Technologies). ..

    Plasmid Preparation:

    Article Title: A molecular framework controlling style morphology in Brassicaceae
    Article Snippet: The Y2H assays were performed at 28°C in the yeast strain AH109 (Clontech) using the co-transformation technique. .. Coding sequences were cloned into Gateway vector GAL4 system (pGADT7 and pGBKT7; Clontech) passing through pDONR207 (Life Technologies). .. Strength of interaction was tested on selective yeast synthetic dropout medium (YSD) lacking Leu (L), Trp (W), Adenine (A), and His (H), supplemented with different concentrations of 3-aminotriazole (Sigma-Aldrich).

    Article Title: SPOROCYTELESS/NOZZLE acts together with MADS-domain transcription factors to regulate an auxin-dependent network controlling the Megaspore Mother Cell development
    Article Snippet: .. AG and STK full-length CDS and SPL/NZZ and SEP3Δ shorter versions (SPL/NZZΔ and SEP3Δ respectively) were cloned into Gateway vector GAL4 system (pGBKT7 and pGADT7, Clontech; pTFT1 ). ..

    Article Title: TEOSINTE BRANCHED1 Regulates Inflorescence Architecture and Development in Bread Wheat ( Triticum aestivum )
    Article Snippet: .. These open reading frames were cloned into the Gateway vector GAL4 system ( pGADT7 and pGBKT7 ; Clontech) passing through pDONR207 (Life Technologies, Thermo Fischer Scientific). ..

    Article Title: BPC transcription factors and a Polycomb Group protein confine the expression of the ovule identity gene SEEDSTICK in Arabidopsis.
    Article Snippet: All rights reserved Yeast two-hybrid assay The two-hybrid assays were performed at 28°C in the yeast strain AH109 (Clontech). .. The coding sequences of BPC4, BPC6 and SVP were cloned into pDONR207 (Life Technologies) and successively transferred to the Gateway vector GAL4 system (pGADT7 and pGBKT7; Clontech). ..

    Article Title: A noncanonical auxin-sensing mechanism is required for organ morphogenesis in Arabidopsis
    Article Snippet: The Y2H assays were performed at 28°C in the yeast strain AH109 (Clontech) using the cotransformation technique ( ). .. Coding sequences were cloned into pGAD424 and pGBKT9 vectors (Clontech) using the SmaI and PstI sites or cloned into the Gateway vector GAL4 system (pGADT7 and pGBKT7; Clontech) passing through pDONR207 (Life Technologies). ..

    Article Title: MADS-Box and bHLH Transcription Factors Coordinate Transmitting Tract Development in Arabidopsis thaliana
    Article Snippet: The two-hybrid assays were performed at 22°C in the yeast strain AH109 (Clontech), using the cotransformation technique ( ). .. The coding sequences of CESTA , STK , and BEE1 were cloned in the Gateway vector GAL4 system (pGADT7 and pGBKT7; Clontech) passing through pDONOR207 (Life Technologies). ..



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    ( A ) Yeast-two-hybrid assay showing interaction between SPL/NZZ and MADS-domain TFs (SEP3, STK, AG). The controls are shown in the . Interactions are tested on media depleted of histidine (-W -L -H), or histidine and adenine (-W -L -H -A). For each interaction on the different media, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( B, C ) Yeast-three-hybrid assay showing the formation of SPL/NZZ, STK and SEP3 complex, by fusing SPL/NZZ either to the <t>GAL4</t> BD ( B ) or to the TFT ( C ). Interactions are tested on a medium depleted of histidine (-W -L -H -A). For each interaction, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( D, E ) pSEP3::SEP3:GFP reporter line showing SEP3 accumulation in ovules at stage 2-I ( D ) and 2-III ( E ). ( F, G ) pSTK::STK:GFP reporter line in ovules at stage 2-I ( F ) and 2-II ( G ). ( H, I ) pAG::AG:GFP reporter line in ovules at stage 2-I ( H ) and 2-II ( I ). Abbreviations: nu= nucella; ch= chalaza; fu= funiculus; ii= inner integument; oi= outer integument; pl= placenta. Scale bar = 20 μm.
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    Image Search Results


    ( A ) Yeast-two-hybrid assay showing interaction between SPL/NZZ and MADS-domain TFs (SEP3, STK, AG). The controls are shown in the . Interactions are tested on media depleted of histidine (-W -L -H), or histidine and adenine (-W -L -H -A). For each interaction on the different media, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( B, C ) Yeast-three-hybrid assay showing the formation of SPL/NZZ, STK and SEP3 complex, by fusing SPL/NZZ either to the GAL4 BD ( B ) or to the TFT ( C ). Interactions are tested on a medium depleted of histidine (-W -L -H -A). For each interaction, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( D, E ) pSEP3::SEP3:GFP reporter line showing SEP3 accumulation in ovules at stage 2-I ( D ) and 2-III ( E ). ( F, G ) pSTK::STK:GFP reporter line in ovules at stage 2-I ( F ) and 2-II ( G ). ( H, I ) pAG::AG:GFP reporter line in ovules at stage 2-I ( H ) and 2-II ( I ). Abbreviations: nu= nucella; ch= chalaza; fu= funiculus; ii= inner integument; oi= outer integument; pl= placenta. Scale bar = 20 μm.

    Journal: bioRxiv

    Article Title: SPOROCYTELESS/NOZZLE acts together with MADS-domain transcription factors to regulate an auxin-dependent network controlling the Megaspore Mother Cell development

    doi: 10.1101/2025.03.11.641985

    Figure Lengend Snippet: ( A ) Yeast-two-hybrid assay showing interaction between SPL/NZZ and MADS-domain TFs (SEP3, STK, AG). The controls are shown in the . Interactions are tested on media depleted of histidine (-W -L -H), or histidine and adenine (-W -L -H -A). For each interaction on the different media, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( B, C ) Yeast-three-hybrid assay showing the formation of SPL/NZZ, STK and SEP3 complex, by fusing SPL/NZZ either to the GAL4 BD ( B ) or to the TFT ( C ). Interactions are tested on a medium depleted of histidine (-W -L -H -A). For each interaction, yeast has been spotted at four different concentrations according to a serial dilution (OD 0,5; 1:10; 1:100; 1:1000). ( D, E ) pSEP3::SEP3:GFP reporter line showing SEP3 accumulation in ovules at stage 2-I ( D ) and 2-III ( E ). ( F, G ) pSTK::STK:GFP reporter line in ovules at stage 2-I ( F ) and 2-II ( G ). ( H, I ) pAG::AG:GFP reporter line in ovules at stage 2-I ( H ) and 2-II ( I ). Abbreviations: nu= nucella; ch= chalaza; fu= funiculus; ii= inner integument; oi= outer integument; pl= placenta. Scale bar = 20 μm.

    Article Snippet: AG and STK full-length CDS and SPL/NZZ and SEP3Δ shorter versions (SPL/NZZΔ and SEP3Δ respectively) were cloned into Gateway vector GAL4 system (pGBKT7 and pGADT7, Clontech; pTFT1 ).

    Techniques: Y2H Assay, Serial Dilution, Hybrid Assay